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Objective@#To explore the adverse effects of high temperature on emergency admissions of children during the summer in Beijing.@*Methods@#Child emergency admissions was collected from 30 hospitals in Beijing during the summer of 2016-2018, as well as data related to meteorological factors and air pollutant concentrations. A generalized linear model was used to analyze the association between the daily mean temperature and emergency admissions of children due to total non accidental diseases, circulatory diseases, and respiratory diseases during the summer in Beijing.@*Results@#During the summer of 2016 to 2018 in Beijing, the daily mean temperature was (24.06±3.59)℃, and the daily mean relative humidity was (65.08±17.45)%. Every 1 ℃ increase in the daily mean temperature on the day of exposure had a significant effect on emergency admissions of children, aged 0-14 years old, due to total non accidental diseases and respiratory diseases in Beijing, such that the risk of emergency admissions increased by 0.21, 0.64 times, respectively. The effect of high temperature on emergency admissions due to circulatory diseases was not significant. High temperature had inconsistent effects on emergency admissions of children from different age groups. Among them, the largest increase in the risk of emergency admissions due to total non accidental diseases was observed among children aged 5-9 years old, while children aged 0-4 were vulnerable to emergency admissions for respiratory diseases, and emergency admissions for circulatory diseases were the highest among children aged 10-14.@*Conclusion@#High temperature had a significant effect on emergency admissions of children during the summer in Beijing. Pediatric respiratory diseases are sensitive diseases that are associated with a high temperature in summer, and greater attention should be given to this issue.
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Introduction@#Cutaneous protothecosis usually presents as pyoderma-like lesions or infiltrating papules and plaques on the extensor side of the extremities. It can be misdiagnosed as eczema, pyoderma, or a fungal infection. Although it has been isolated from a swimming pool, sewers and rivers in the Philippines, there has been no reported case of cutaneous protothecosis in the country.@*Case summary@#A 78-year-old Taiwanese male farmer visited the dermatology clinic due to a six-month history of a large, pruritic erythematous plaque studded with papulopustules on his left forearm. A potassium hydroxide (KOH) examination showed negative for hyphae or spores. And a skin biopsy showed morula-like bodies, which were highlighted by the Periodic acid-Schiff stain.@*Conclusion@#We report a case of cutaneous protothecosis from Taiwan so Filipino dermatologists will be aware of the clinical and histopathologic manifestations and management of cutaneous protothecosis.
Subject(s)
Skin Diseases, Infectious , Administration, CutaneousABSTRACT
Objective: To study the effects of extracts from Aconitum kongboense on gut immunity of Drosophila melanogaster. Methods: Flies fed with standard cornmeal-yeast medium were used as control, and the treatment groups contained 1% of A. kongboense aqueous extracts in standard medium. D. melanogaster was treated by some toxic compounds such as sodium dodecyl sulfate (SDS), DSS, H2O2, and Paraquat (PQ) with or without extracts of A. kongboense, and the effect of A. kongboense on the survival rate, intestinal epithelium cell death, relative contents of reactive oxygen species (ROS) in intestinal epithelium cells, and the number of intestinal epithelium cells and intestinal morphology changes were analyzed. Results: A. kongboense could significantly improve the survival rate of SDS, DSS, H2O2, and PQ-infected D. melanogaster, reduce the intestinal epithelial cell death and the level of intestinal ROS, decrease the over-proliferation of intestinal epithelial cell and the formation of melanotic masses. Conclusion: The extracts of A. kongboense could significantly improve the injury induced by chemical substances. This result provides experimental foundation for the exploitation of A. kongboense.
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<p><b>OBJECTIVES</b>To examine the gene expression profile of bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor (VEGF) during entochondrostosis of mice and explore the expression rules and effects between BMP-2 and VEGF, and to detect the expression of VEGF in BMP-2 induced entochondrostosis in vivo.</p><p><b>METHODS</b>cDNA microarray technique with 34,000 genes was used to analyze the gene expression profiles during entochondrostosis in the limbs of mice embryo from E10 to E14. Pathway analysis of BMP-2 and VEGF was performed with GCOS1.2 software. An experimental model of femoral muscular pouch in 20 mice was adopted. The expression of VEGF was examined by in situ hybridization method and immunohistochemical method in BMP-2 induced entochondrostosis in vivo.</p><p><b>RESULTS</b>The expression signals of VEGF mRNA and VEGF appeared in cytoplasm during condensation of mesenchymal cell. As the mesenchymal cells differentiated into precartilage, the expression signals decreased in mesenchymal cells, but increased in chondrocytes and kept getting denser in the process of cartilage maturity. The peak expression of VEGF mRNA and VEGF in the experimental group appeared on the 14th day, accompanied by numerous hypertrophic chondrocytes. When mature cartilage calcified and new bone trabecula formed, the expression of VEGF mRNA and VEGF decreased in chondrocytes, but still expressed moderately in the osteoblasts and osteocytes.</p><p><b>CONCLUSIONS</b>The finding reveals a complex pattern of gene coexpression of BMP-2 and VEGF during the critical period of entochondrostosis. It's feasible for the clinical application of BMP-2 in orthopedics.</p>